544 LIGHT AND LIFE 



TABLE 11 

 Effect of o-i-iiiNAMHROLiNE and CMU on Cyclic: Photophosphorylation 



IN Nitrogen or Air 

 (TsujiMOTO, Hall, and Arnon, 156) 



Inhibitor added 



CMU o-Phenanthroline 



/imoles P Percent junioles P Percent 



Treatment esterified Inhibition esterified Inhibition 



In the nitrogen series the illumination was 2000 Lux for 30 min and the reaction 

 mixture included, in a final volume of 3 ml, chloroplast fragments (C^) containing 1.5 

 mg chlorophyll and 0.3 /umoles of FMN or vit. K3. In the air series the illumination was 

 50,000 Lux for 5 min and the reaction mixture included chloroplast fragments (Cul 

 containing 1 mg chlorophyll and 0.003 /^moles of FMN or vitamin K3. The final 

 inhibitor concentrations were, 3 X 10"*^ M for o-phenanthroline and 2 X 10~^ M for 

 CMU. Other common components of the reaction mixture were, in micromoles: Tris 

 buffer, /;H 8.3, 80; K,H'-PO„ 15; ADP, 15; and MgSO,. 5. In the air series, the 

 photophosphorylation in the control and "inhibitor added" treatments was sub- 

 tracted from that in the other treatments when cakidating the per cent inhibition. 



TABLE 12 



EfFICI of CllI.ORII)! ON CYCLIC IMlOTOPIIOSl'IlORYLATION IN NiTROClKN OR AlR 



(Tsiijimoto, Hall and Arnon, 156) 



Aiinoles P esterified 



Trcalinoil minus chloride I'l'is chloride 



Nitrogen, FMN 5.1 5.7 



Nitrogen, vit. K, 9.7 9.9 



Air, FMN 0.5 fi-l 



Air, vit. K3 0.4 



5.5 



Experimental conditions as in Table 11, except that chloroplasts were prepared 

 in 0.5 M sucrose and chloride-free reagents were used. 0.2 mg chlorophvU was used 

 in the air series, and '.^.5 mg chlorophyll per vessel was used in the nitrogen series. 

 The reaction was run for 30 minutes. 



