F. I. TSUJI, A. M. CHASE AND E. N. HARVEY 



151 



was no cysteic acid, urea, hydroxyproline, citrulline, cystine (nor 

 cysteine), alpha-amino-n-butyric acid, and glucosamine. The relative 

 abundance of the amino acids is shown in Figs. 6 and 7. 



In addition, hydrolysis studies were carried out on luciferin isolated 

 by both paper electrophoresis and paper chromatography. However, 



2.50 - 

 t.ti- 



t.oo- 



I.T» 

 I. SO ■ 



O i.oof- 



to 



O.TS 



< 



q: 



e> 

 o 



Alanine (4 «7I 



5 «»TT...i.. ""•' 



(O o.ts 



Tkrttnint Clalinile ClfCint 



(0.3» Aci4IS.IT) (l.tS) 



Swln«(0.t9) 



" frtlllt 

 S«rc«>ln» I \ i\ (4.l«) 

 10. St) 



AtMrtic 

 Aci4 



<0.I7| 



90 



litltuclii 

 (l.»») 



VtllM 

 (I 4*1 Mtlllionint 

 (O.SO 



ISO 



-»HS.S7, ST.S'e- 

 citratt 



Llnciat 

 (2 1*) 



PhtnylaUnin* 

 (OtI) 



ITS ZOO 229 2S0 2TS JOO S2S 



f. 



ISO 



'»H4.2S,S0 C- 

 cilrttt 



^^»M4.2»-. 

 TS*C 



l.tf 



I.I 



oieo- 

 o.te 



Aaatii* 



< / )ti«il<l«« 7 



Ttrfl«>l>«n« T 



LftlM 



ArfUli*t 

 ' - ■ 



jts 400 4tS 4S0 47S ioO tti »»0 JT9 Soo MS Sso iT» 



TOO 



^»H4.tI->^»H«.T0>- 



r»*c tf'c 



•itt4t* 4itt(tt 



-»n t.io- 



-»H •.tO.tS'C' 



->HII.O, is'c- 



(•r»*4<t* ••f»tMl» 



EFFLUENT cc. 



Fig. 6. Isolation of amino acids from luciferin hydrolyzate by chromatography on 

 Dowex 50 column 0.9 X 100 cm according to the method of Moore and 

 Stein (1951). The column was operated in the sodium form. The pH, the 

 buffers used as eluants, and temperature of operation of the column are 

 indicated. 



only very small amounts of luciferin (4 mg) were obtainable by these 

 methods and, consequently, composite samples had to be used. The 

 luciferin from these two procedures had, as a solid, a characteristic 

 reddish-orange color but was yellow in methyl alcohol solution. These 

 composite samples did not have the clear-cut ultraviolet absorption 

 bands characteristic of the purest luciferin but, rather, showed some 

 general absorption in the ultraviolet range. Compositing many sam- 

 ples by elution from a large number of paper strips evidently caused 

 some contamination. 



