212 BIOCHEMISTRY OF BACTERIAL LUMINESCENCE 



DPN, TPN, DPNHo, riboflavin, FMN, FAD, Co A, lipoic acid, thia- 

 min pyrophosphate, ATP, ADP, AMP, glucose-6-P, glucose, fructose, 

 phosphoglyceric acid, a-ketogkitarate, acetate, acetyl phosphate, cit- 

 rate, malate, succinate, lactate, ethanol, acetaldehyde, vitamin A, 



II 13 15 17 19 21 23 25 



TIME(MIN.) 



Fig. 1. Time course of luminescence emitted by bacterial extracts. A, 2 mg/ml 

 acetonized bacterial powder added at zero time; B, 20 mg/ml acetonized 

 bacterial powder added at zero time; C, 20 fig/ml DPNH; added at zero 

 time; D, 16 Mg/ml DPN added at zero time. Water solution, temperature, 

 23° C. 



pyridoxine, ascorbic acid, glutathione, cysteine, glycine, versene, vita- 

 min K, 2-methyl-l, 4-naphthoquinone, vitamin E, benzoquinone, hy- 

 poxanthine, xanthine, vitamin D, numerous steroid hormones, hemo- 

 globins, a variety of vitamin-rich concentrates, yeast extract, Mg+ + , 

 Fe++, Mn++, Pb++, Zn++, Co,+ +, etc., and cytochrome c. Most of 

 these were tested not only alone, but in combination with other com- 

 ponents. 



Of the compounds examined only the following showed marked 

 effects: DPN, DPNH2, riboflavin, FAD, FMN, naphthoquinone, Co A, 



