BERNARD L. STREHLER 



225 



50- 



K^(PAL.l=0.55mm 

 Km(N0W4L.)=2.03mm 



-200 



o 

 150| 



5 



3 



lOOS 



O 



z 

 o 



50 



o 

 o 



5 10 15 



CONCN OF OXYGEN (mm) 



Fig. 10. Effect of o.xygen concentration on luminescence of bacterial extracts in 

 the presence and absence of palmital. To 1.0 ml of a 10% aqueous extract 

 of A. jischeri in 0.1 M phosphate buffer (pH 7.0) was added 2 mg of DPNHa 

 and 8 fig of FMN. Oxygen tension was measured with a dropping mercury 

 electrode simultaneously with the light emission. 60 ng of palmitic aldehyde 

 was then added to the same aliquot of extract and oxygen tension and lumi- 

 nescence were again measured. Total \olume 4.0 ml. # No palmital. O 

 with palmital. 



of these present experiments was to add all except one of the com- 

 ponents necessary for a bright luminescence to the enzyme and then, 

 at zero time, to add the remaining necessary component. The light 

 output as a function of time after mixing was measured and recorded 

 electronically and the approach to steady-state luminescence was 

 plotted in such a manner as to give an index of the % rise time, i.e., 

 the time required for the luminescence to become half maximal after 

 mixing the various components. It was found that the terminal addi- 



