BERNARD L. STREHLER 



lO-A 



227 



SHORT SCALE 



O 02ADD'N 

 • PAL. ADD'N 



A DPNH2 ADD'N | LONG SCALE 

 A FMN ADD'N 



TEMP. =22°C 



4 6 8 



TIIVIE AFTER MIXING (SeC.) 



Fig. 11. Plot of data on half-rise times for oxygen, palmital, DPNHj, and FMN 

 when each of these factors is added last to an enzyme preparation contain- 

 ing an excess of the other factors. The extrapolated maximum value for 

 luminescence was taken from the records (Rmax). The values for lumines- 

 cence at various times after mixing the last necessary component were sub- 

 tracted from Rni.ix and plotted on a logarithmic axis against the time after 

 mixing. 



