BERNARD L. STREHLER 



231 



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z 



UJ 



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3 



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UJ 

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4= 8000 PS. I. ON 

 |=PRESSURE OFF 



.+ XS KCF 



MINIMAL 

 KCF 



NO KCF 



f 



\ 



FMNHi 



OPNH. 



NO LIGHT 



ADDED HERE 



3 4 

 TIME 



6 7 8 



(MINUTES) 



9 10 



Fig. 13. Idealized representation of tlie effects of hydrostatic pressure on bacterial 

 extract luminescence. Reading from the top down, the figure illustrates the 

 effect of pressure application and release in the presence of excess decalde- 

 hyde (ca. 10 ;ug/ml); in the presence of limiting concentrations of aldehyde 

 (ca. 0.37 jtig/ml); in the absence of added aldehyde; when FMNHa is the 

 substrate ( aldehyde present ) ; and finally ( for comparison with the top 

 curve), the time course of luminescence when DPNH2 (or FMN) is added 

 last. Temperature ca. 16° C. 



