LIVING SPIROCHAETES 



17 



organism. The parasite continued in this position for five 

 minutes. Another Spirochaete was in the « cyst )> condition for 

 three minutes. Neither organism remained permanently in this 

 phase, however, but each uncoiled suddenly and swam away, 

 apparently unchanged. I suggest that had Perrin lîxed his 

 material at this coiled up phase and had then stained his pre- 



FiGS. 25-28. Pseudo-encystment fonns. 



Fio. 25. Normal form. 



FiG. 26. Form with loose, central coils. 



FlQ. 27. The same with tightened coils (pseudo-cyst.). 



FiG.'^28. Organism uncoiled again. 



para tions,^ the effet would be that shown in his figures. Should 

 this be the case it serves to emphasise the great need of lengthy 

 observations of the living organisms. 



I may say that the prolonged examination of several species 

 of hving Rpirochaetes affords no évidence of socalled conjuga- 

 tion between socalled sexual forms. 



ARCH. DE ZOOL. EXP. ET GÉN. — 5« SÉBIE. — T. III. — (I). 



