12 ANNIE PORTER 



For longer distances the organisms move by the agency of 

 the myonemes of the body and membrane in the manner des- 

 cribed by Fantham (I. 1908, pp. 16-25). 



The movements of the Spirochaetes that I hâve examined 

 from Mytilus and Sphaerium are very like those of S. halhianii 

 and S. anodontae. 



The remarkable movements exhibited by Spirochaetes during 

 division are considered imder that section. 



Proof of the spiral path of a Spirochaete is revealed by the 

 passage of the organism through any stained médium in which 

 it is immersed. By its spirally boring movement, the denser 

 liquid is displaced and a spiral, wavy, relatively unstained path 

 results. As a conséquence of this, the organism fînally lies as a 

 coloured slender thread, within a colourless, spirally curved 

 area, formed by its own movement. Jaffé also noted the clear 

 area around the Spirochaetes of Culex in the stained prépara- 

 tions which he examined. 



V. — Multiplication and reproduction. 

 A. Division 



Some two years ago, when I had just begun this séries of 

 further observations on Spirochaetes, I was told by an Italian 

 friend (the late Signor Bernardi), that the mode of division of 

 Spirochaetes needed re-investigation, for what had been des- 

 cribed as longitudinal division was merely the resuit of the 

 folding of the organism vipon itself. Swellcngrebel has since 

 published to the same effect, stating that transverse division 

 occurs and that folding or entanglement of organisms may 

 simulate longitudinal division 



In the course of my investigations, I hâve frequently seen 

 a Spirochaete doubly coiled on itself (fîgs. 9-12). To the un- 

 wary this might at first suggest longitudinal division. But the 



