138 The Ottawa Naturalist. [September 



gations regarding fatty degeneration by Wettick, Forster and 

 others, confirmed Voit's results. 



Dumas and Milne Edwards jointly instituted an experiment 

 with bees to find out out whether fat can be produced from fat 

 free substance. 



A swarm of bees were fed with honey for 32 days with the 

 following results : — 



Amount ot wax produced 11. 515 grammes. 



II lat in honey 0.667 " 



II fat produced from fat free 



substance 10.848 n 



This opened their eyes to the truth of Liebig's statement and 

 they acknowledged that fat could be formed from fat free sub- 

 stance. With Voit they supported the view that it was derived 

 from the pre-existing fat and the transformed protein matter. 



Other experiments were therefore instituted with the especial 

 object of ascertaining whether or not carbohydrates play a part in 

 the formation of fat. 



Berlepsh experimented with bees feeding them on 1 17 grammes 

 of pollen and honey. The 117 grammes of pollen contained 22 

 grammes of protein : — 



22 grammes protein = at the most 12 grammes wax. 



Amount of wax produced by the bees • • • 33 '* 



11 11 from other sources 21 " 



Possible amount of wax in bodies of bees. 10 " 



Amount of wax necessarily formed from 



carbohydrates 11 grammes. 



E. Erlenmeyer, in 1878, wishing to prove conclusively that the 

 fat could come from carbohydrates alone, fed a swarm of bees 

 solely on rock candy. From each 8 grammes of sugar consumed 

 there was produced 1.589 grammes of wax, which could not have 

 possibly been formed from protein. The nitrogen and fat content 

 of the bees remained unchanged during the experiment. 



Henneberg, Kern and Wattenberg experimented with sheep : 



A sheep was fed for 70 days with lucerne hay, maize meal and 

 turnips : — 



