362 



Relation of "Water to the Behavior of 



By consulting this figure, one can see at a glance that the curves for trans- 

 piration and evaporation correspond. Moreover, the reciprocal of the reaction 

 or behavior curve also corresponds to these curves in a similar manner. Thus 

 in the reaction of the potato beetle, its percentages of positiveness was, broadly 

 speaking, the reciprocal of the transpiration curve. This seems to show that the 

 loss of water from the animal, when exposed in the open, determined the reac- 

 tions of the insect. 



Table 8.- 



-Results of subjecting other insects to the same environmental conditions 

 as Leptinotarsa decemlineata. 



In- 

 sects. 



Time. 









So . 



™ '" '3 

 3 * J 



X 



General Remarks. 



10 a. m. 



11 a. m. 



1 p. m. 



2 p. m. 



3 p. m. 



4 p. m. 

 6 p. m. 



6 p. m. 



7 p. m. 

 10 p. m. 



C a. m. 

 10 a. m. 



10 a. m. 



11 a. m. 



1 p. m. 



2 p. m. 



8 p. m. 



4 p. m. 



5 p. m. 



6 p. m. 



7 p. m. 

 10 p. m. 



6 a. m, 

 10 a. m. 



10 a. m. 



11 a. m. 



1 p. m. 



2 p. m. 



3 p. m. 



4 p. m. 



5 p. m. 



6 p. m. 



7 p. m. 

 10 p. m. 



10 a. m. 



11 a. m. 



1 p. m. 



2 p. m. 



3 p. m. 



4 p. m. 



5 p. m. 



6 p. m. 



7 p. m. 

 10 p. m. 



gms. 

 7.2700 



6.7025 



6 ! 4970 

 6.3620 

 6.1735 



2.1790 



1.5715 



1.0545 

 0.8030 



8 ] 2.9510 



2.7845 



1 .3055 

 1.2705 



5.1725 



4.6005 

 4!6346 



gmi. 

 0.0000 



2690 



2056 

 1350 

 1885 



0000 



0575 

 0290 



1666 



0880 



0380 

 0350 



0000 

 3462 



2258 

 1690 



gm». 

 0.0000 



%H^0 

 0.00 



7.90 

 5.19 

 7.26 



0.00 



22.16 



10.23 

 7.30 



18.79 

 'i4!42 



"^'.i'i 



9.07 

 0.00 



27'.54 



17.96 

 4!ii 



%B^0 

 0.00 



2.77 



1.98 

 0.65 

 1.81 



2.56 

 0.91 



4.70 



2!46 

 1.13 



0.00 

 6!89 



3.53 



No. 



9 



10 



10 



10 



10 



10 



10 



10 



10 

















 

 

 

 

 

 

 

 

 

 

 





 

 

 

 

 

 

 

 

 



p. ct. 



90 



100 



100 



100 



100 



100 



100 



100 



100 

















 

 

 

 

 

 

 

 

 

 

 





 

 

 

 

 

 

 

 

 



All doad. 



3 dead ; deduct 0.235 gm, 



5 dead = 0.4695 gm. 

 2 dead = 0.2225 gm. 

 All dead. 



2 dead = 0.7525 gm. 

 2 dead = 0.6005 gm. 

 All dead. 



1 dead = 0.4075 gm. 

 All dead. 



The second experiment was performed to determine the relation of evapora- 

 tion to the transpiration and reactions of insects when exposed for several days 

 under natural conditions. To get a comparison between L. decemlineata and 

 other insects, a cricket {Gryllus), a beetle {Catalpa lanigera), and two species 

 of June-bugs (Lachnosternae) were used, since they could be collected in large 

 numbers. These insects, with the exception of the potato-beetles, were obtained 



