remainder with rodents. More than half of the papers are of direct interest to develop- 

 mental immunologists. 



The volume is well printed and illustrated. 



METHODS (see also 9,43,1 13,115) 



Monographs 



119. 



J. A. LAST, ed. 1976. EUKARYOTES AT THE SUBCELLULAR LEVEL, development 



and differentiation 



Dekker, New York, etc. Methods in Molecular Biology, Vol.8. X, 460pp., 31 figs., 



1 1 tabs., author and subject indexes. $ 32.50, SFr. 1 15.00 



This book is a compendium of sophisticated methods in molecular biology that will 

 certainly prove most useful to developmental biologists. It contains a wealth of well- 

 organised practical information. Apart from a general chapter on nucleic acid hybridisa- 

 tion the book is organised around six widely divergent developing systems which are 

 either known in detail or hold great promise for the future. Many chapters provide a 

 description of the protein inventory of the system and all but one describe the techniques 

 for the isolation, purification and further analysis of messenger RNA. 



The following systems are considered: the insect chorion (by Kafatos), cellular slime 

 moulds (by Jacobson), erythroid development (by Tobin et ai), lung collagen (by Cowan 

 et ai), chick embryo myosin (by Sarkar), and the chick oviduct (by Rosen et ai). 

 Immunoglobulin mRNA is not considered, and no details are given of the immuno- 

 precipitation method for the purification of polysomes. The chapters are adequately 

 cross-referenced. 



The book is produced in readable offset print. The subject index is somewhat Umited. 



Symposium reports 



120. 



O. MiJHLBOCK, ed. 1976. BASIC ASPECTS OF FREEZE PRESERVATION OF 



MOUSE STRAINS (Proceedings of a Workshop) 



G. Fischer, Stuttgart. X, 133 pp., 36 figs., 42 tabs. DM 48.00 (paper) 



Although freeze preservation of mammalian embryos was developed for other than 

 embryological purposes, embryologists may be interested in this report. The workshop 

 was held at the Jackson Laboratory in Bar Harbor, Maine in September 1974 and was 

 attended by investigators from the U.S.A., England and the Netherlands. 



A series of 11 brief reports treat various aspects of this new method. At least half of 

 them contain methodological and other data that may be of interest to embryologists and 

 developmental geneticists. More similar information is to be found in the discussions. 

 Many "tricks of the trade" are extensively discussed. One report deals with the effects 

 of ionising radiation on pre-implantation mouse embryos in vitro, and another with 

 immunological aspects of maternal influences. 



121. 



Th H SHEPARD J. R. MILLER, and M. MAROIS, eds. 1975. METHODS FOR DETEC- 

 TION OF ENVIRONMENTAL AGENTS THAT PRODUCE CONGENITAL DEFECTS 



North-Holland, Amsterdam, etc.; Amer. Elsevier, New York. 263 pp., 58 figs., 27 tabs., 

 subject index. $ 34.75, Dfl. 90.00 



This is predominantly a book of practical information and as such wUl be indispen- 

 sable to many teratologists in their everyday work. The conference, held in Guadeloupe, 

 West Indies in January 1974 and sponsored by the "Institut de la Vie", brought together 



215 



