J. R. GREGG 255 



Table I. Phosphorus Metabolism of R. pijnens and Hybrid Embryos 



Source: Reconstructed from Barth and Jaeger, 1947, Table 7, together with 

 original data kindly supplied by these authors. 



Embryos were allowed to develop 10-22 hr. in aerated or nitrogenatcd me- 

 dium, beginning at Stage 10, after which phosphorus fractions weie measured. 



Total This is essentially the total inorganic phosphorus in a trichloracetic 

 acid extract after 7 min. hydrolysis in INHCl at 100° C. 



I This is essentially the total inorganic phosphorus in a trichloracetic acid 

 extract before acid hydrolysis. 



E7 = Total — I, and is interpreted to be mostly inorganic phosphorus liber- 

 ated by acid hydrolysis from ATP and ADP. Values are in micrograms per 

 100 embryos. 



s = standard deviation = ' "^ 



\n - 1 



Probabilities of differences determined by t-tost for small samples, where 



_ X — m.o 



s/Vn 

 Throughout, n = 4. 



hybrid increase in I is shown not to be significantly different from 

 that of R. pipiens embryos, but is in excess of that which might 

 be expected to occur as the result of the liberation of I from 

 breakdown of E7. 



Thus, if inorganic phosphorus is a reliable indicator, anaerobic 



