S. RANZI 



301 



20 





10 



10 20 30 40 50 60 70 80 90 C 



Fig. 10. Graphs of salting out plotted following Derrien et al. for frog 

 embryos at stage lOM left for 6 hours in Holtfreter solution (control), or 

 NaSCN, or LiCl. (From Ranzi and Citterio, 1955.) 



ment with LiCl, a higher amount of fractions precipitating at a 

 lower concentration of ammonium sulfate (30 to 50% saturation) 

 appears. In the embryos treated with NaSCN, a greater amount 

 of proteins precipitate at a higher concentration of ammonium 

 sulfate (55 to 60% saturation). 



Embryos after a one-day treatment with NaSCN develop with 

 large notochords and have a far greater quantity of proteins pre- 

 cipitating at higher concentrations of ammonium sulfate (Ranzi 

 and Citterio, 1955a) (Fig. 11). 



Contr 



10 20 30 40 50 60 70 80 90 C 



Fig. 11. Graphs of salting out like those of Fig. 10 showing NaSCN 

 action. Controls at stage 12. (From Ranzi and Citterio, 1955.) 



