Organ Culture Studies of Reproductive Tracts 13 



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DISCUSSION 



Jost: I was very much interested in Dr. Price's magnificent results 

 and very glad, I must say, to see in what good agreement they are with 

 some personal findings. It seems clear that in the absence of the testes, 

 the Wolffian ducts do not persist and develop. This seems to be true in 

 the rabbit, in the rat and most probably in man also, since it is well 

 known that in cases of gonadal agenesis (so-called "ovarian agenesis") 

 no Wolffian duct persists. 



Some years ago, we tried in my laboratory to confirm by experiments 

 in vitro the results obtained in vivo in castrated rabbit foetuses. I should 

 like to show you two slides taken from former papers (Jost, 1950; Jost 

 and Bozic, 1951). Small pieces of the genital tract containing both the 

 Wolffian and the Miillerian ducts (rat embryo on day 16£) were culti- 

 vated in serum of castrated or non-castrated adult rats. In the next 

 three days whatever the sex of the embryo the Wolffian ducts retro- 

 gressed and disappeared while the Miillerian ducts developed and pro- 

 liferated; so the ducts behave in vitro as they do in vivo in castrated 

 rabbit foetuses. These results seem to be in good agreement with 

 Dr. Price's more extensive experiments. 



But, Dr. Price, you did not discuss the point of the Miillerian ducts. 



