THE PITUITARY BODY 



which the preparation used is metaboHzed, possible syner- 

 gistic or antagonistic effects of substances in the extract, etc. 

 There is increasing recognition of the inabihty to control the 

 effects of the anterior pituitary secretion if normal animals 

 are used for assay. Especially is it necessary to use hypophy- 

 sectomized animals in testing extracts considered to have 

 specific qualitative effects. 



Investigations of the relationship between dosage of pitui- 

 tary gonadotropic hormone and the response of the gonads 



012 024 03« 



Fig. 14. — The effect of various doses of follicle-stimulating hormone of "cas- 

 trate" urine on the weight of the ovary and uterus of the mouse. (From Levin and 

 Tyndale, Endocrinology, 21, 619-28 [1937].) 



have been made recently by Deanesly (1935), Chow and Liu 

 (1937), and Levin and Tyndale (1937). Deanesly recom- 

 mended that the ovarian hypertrophy produced for quantita- 

 tive assay of pituitary gonadotropic hormone should be repre- 

 sented by paired ovarian weights within a range of about 30- 

 80 mg.^'' However, it has become increasingly clear that the 

 use of ovarian weight as a basis for assay may have only a 

 limited value. Levin and Tyndale found that the response 

 of the uterus of the immature albino mouse to the follicle- 



^ After fixation in Bouin's fluid and partial dehydration (70 per cent alcohol). 



1 108 1 



