NORTHEY 



Figure 2. Starch gel electrophoresis of sera from "cold exposed" (bottom of picture) 

 and "non-cold exposed" (top of picture) rabbits. 



ratus of our own design which utilized a borate buffer having an 

 ionic strength of 0.01 in the gel and an ionic strength of 0.0 3 in the 

 borate bridge. In all cases, samples were analyzed simultaneously 

 using a plastic trench which accommodated two samples at once. 

 A piece of filter paper was saturated with the serum and placed in a 

 slit in the gel. Samples representing both the "cold stressed" 

 samples and "normal" samples were run side by side at a current 

 of 250 volts 5 ma per sample for a five hour period. At the end of 

 the migration period, the strips were cut and stained with amido 

 (black) Schwartz. Permanent records were maintained by sketching 

 the pattern on graph paper and by photographs. A representative 

 starch gel strip is shown in Figure 2. 



Thus far, a total of more than 300 serum samples from "cold ex- 

 posed" and non-cold exposed animals have been analyzed by starch 

 gel electrophoresis, and a critical evaluation of the data has failed 

 to reveal any striking differences. The presence of any "abnormal 

 proteins" in the sera of cold exposed animals was not detected. 



However, a serum fraction, which has tentatively been identified 

 as one corresponding to the B lipoprotein of paper electrophoresis 

 (the alpha- 2 lipoprotein of immuno- electrophoresis), has been 

 found to be weak or absent in a number of serum samples from 



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