IMMUNE RESPONSE IN COLD EXPOSURE 



Undilute 

 Antigen 



1- 



I - 2 



Rabbit 

 anti- serum 



1 - 4 



Figure 1. CXichterlony gel diffusion analysis of bovine serum-- rabit antibovine 

 serum. 



Shannon Scientific Company of London, and distributed in this 

 country by Consolidated Laboratories. This pattern consists of a 

 center well surrounded by four wells equidistant from it. These 

 four wells were used for the various antigen dilutions. In order to 

 approximate more closely the region of optimal proportions, four 

 samples of antigen were tested in each case; that is, undilute, 1:2, 

 1:4, and 1:8. Plates were read at appropriate intervals in order to 

 determine the time of precipitation of the first reacting components, 

 and the results of each of the readings were recorded on master 

 sheets designed for this purpose. Readings in all cases were taken 

 from the antigen- antibody concentrations which gave the most defin- 

 itive results. A sample plate is shown in Figure 1. 



For the data obtained to be best evaluated, it became necessary 

 to adopt a more accurate method of measurementbased on statistics. 

 The method of choice was the Mann- Whitney U test. This nonpara- 

 metric test is a useful alternative to the parametric "t" test when 

 the measurement in the research is weaker than interval scaling. 



Aladjem et al,(1959) have reported that the time lapse preceding 



113 



