TRAPANI 



fornia. Some were immunized in Pasadena before going up, 

 some were immunized when they first got there, some were 

 immunized after seven days, some after a month, and some 

 were just brought up there for a month, then immunized and 

 returned to Pasadena. The only group which seemed to give 

 a fairly clear picture so that we could say, "Well, let's study 

 this one because it's a little simpler," was the group which 

 was brought to a high altitude, adapted for thirty days and then 

 immunized, and the immune response studied while the ani- 

 mals were kept at high altitude. The variation of response in 

 the other groups was too great to merit further study at the 

 time. 



PREVITE: I am wondering what would be the antibody re- 

 sponse to cold stress within the first few days if one used a 

 rabbit with a known titer ? Would you expect antibody titers to 

 decrease? 



TRAPANI: By inference, I would say antibody levels may 

 go down; these are given levels of circulating antibody. When 

 the animal is put into the cold, the metabolic rate is increased 

 to overcome the heat load; antibody levels might then decrease 

 as a consequence of an increased turnover rate. 



PREVITE: It seems that most of us agree that normally we 

 are warmly clothed when we are outdoors in cold weather. May- 

 be one is only accidentally going to be stressed by the cold en- 

 vironment. Perhaps it is most important, in analysis of the 

 effects of cold exposure on infectious diseases, to determine 

 the response to this chance exposure. 



TRAPANI: I would like to make one more comment that Dr. 

 Berry brought to mind. I think the antigen must be considered 

 not only in terms of its persistance, but also in regard to its 

 chemical nature. The system I used was a soluble one of BSA 

 and anti-BSA. If one uses a viral or bacterial system, these 

 different kinds of antigen might be handled differently by the 

 animals under these situations. Dr. Berry has shown that in 

 animals subjected to high altitudes, resistance to bacterial in- 

 fection is decreased, whereas resistance to viral infection is 



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