IMMUNIZATION OF STRESSED ANIMALS 



by the cell. There is a possibility, if antigen acted as a tem- 

 plate, that it may momentarily be associated with the antibody, 

 but actually would immediately disassociate, or disassociate 

 soon after it got out of the cell, probably inside it. There is no 

 question but that antigen breakdown and loss is associated with 

 antibody formation. 



MONCRIEF: Not necessarily the other way around? 



CAMPBELL: No. 



MONCRIEF: Do you know anything about the diet of the hiber- 

 nating animal with respect to protein, carbohydrate, and fat 

 composition that these people on Ladd Field worked with when 

 they measured clotting time? 



CAMPBELL: The hibernating animal, of course, isn't eating. 



MONCRIEF: Prior to his going into hibernation. 



CAMPBELL: He is in pretty good shape under natural con- 

 ditions. I don't know what all they do eat, besides berries and 

 roots, and so on. 



MONCRIEF: The only reason I ask, is that a very peculiar 

 observation came up about a year ago; Walter Blum was put- 

 ting patients on starvation diets. These patients were placed 

 on a completely carbohydrate-free diet, nothing but fat and pro- 

 tein; he drew blood samples from these patients and placed them 

 in the freezing portion of the ice box, and a few weeks later 

 when I happened to be visiting him, he took samples out to show 

 them to me. He pulled about twenty samples of blood out of 

 the refrigerator, six of which were from patients on this diet. 

 The other fourteen were frozen solid, but the six on this diet 

 were still completely liquid. He later analyzed these for every- 

 thing he could consider possible and found nothing to be ab- 

 normal in the blood except an elevation of the non- ester if ied 

 fatty acids. Even serum osmolarity was the same. 



CAMPBELL: I forgot to mention, these sera that don't clot, 



87 



