CAMPBELL 



high incidence of respiratory diseases among the Marines in spite 

 of immunization. We suspect that lack of resistance was due to the 

 rapid metabolic turnover of Ab protein. 



Shown in Figure 1 is a regular elect rophoretic pattern of awake 

 and hibernating squirrels. The hibernating squirrels show a higher 

 amount of protein than the awake squirrels which may reflect a 

 loss of blood volume during hibernation. Note the fast- moving com- 

 ponent which developed in a lot of the hibernating squirrels. The 

 fibrinogen, if it is there, would probably be in the beta component. 

 It is very difficult to interpret these data because of the extreme 

 abnormality of the physiological state of the animal. 



Figure 2 shows a comparison of serums taken in January from 

 healthy volunteers at Ladd Field in Alaska. A peculiar thing was 

 that the beta anomaly, usually occurring inthe descending boundary 

 of practically all normal human serums in the temperate zones, 

 was absent. I'm not sure that this is significant, but this beta 

 boundary disturbance is in some way associated with lipoproteins. 

 In the summer time most serums showed this component. In the 

 first studies, it was concluded that serums from these men did have 

 a higher clotting time in winter than in the summer. I never saw 

 the actual data which should be more carefully studied. 



Figure 3 presents data on the retention of antigen in the livers 

 of normal and immunized rabbits. I have included it to show that 

 antigen in the normal rabbit will persist for a long period of time 

 in the liver, and can be detected up to 350 days after the last 

 injection, thus making it a baseline. Recent work by Jerislav 

 indicates that the antigen persists a lot longer in hibernating 

 squirrels. 



Experiments designed to determine if high altitude and low 

 temperature had any effect on experimental asthma in guinea pigs 

 were conducted by Dr. Heimlich and Dr. Trapani at the White 

 Mountain laboratory. The results indicated that if guinea pigs were 

 sensitized at room temperature and sea level, they were quite 

 resistant to challenge. Subsequent studies suggested that this re- 

 sistance was apparently a stress phenomenon, and if the guinea 

 pigs were allowed to adapt for a few weeks, they reacted like 



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