NORTHEY 



Figure 3. Immuno-electrophoretic analysis of rabbit anti-human serum antibody in 

 "cold exposed" (top of picture) and "non-cold exposed" (bottom of picture) rabbits. 

 1. Barbitol buffer strength ionic strength 0.375. 2. Stain- thiazine red R. 



Quantitative Precipitin Analysis 



A limited number of serum samples have been analyzed by the 

 micro-quantitative precipitin method of Lanni (Lanni et aU, 1950). 

 The results of this analyses are given in Table VIII, 



Cold Exposed 

 Non-Cold Exposed 



Antibody Protein 

 mg/ml 

 0.U28 + 0.15H8* 



0.3716 + 0.0767 



Table VIII. ^Quantitative precipitation analyses of sera from "cold exposed" and "non- 

 cold exposed" rabbits. 



It may be noted from the table that the cold exposed rabbits 

 gave slightly higher levels than the non-cold exposed group. This 

 finding is in accord with that of Trapani and Campbell who noted 

 somewhat higher levels in the plasma proteins of cold exposed 

 (1° C to -15° C) rabbits (Sutherland et al., 1958). 



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