Figures 50-72. — Technic artifacts in erythrocytes. 2,470X. 



50 Late polychromatic erythrocyte with internal frac- 

 turing of the cytosome. Probably occurs as the 

 slide dries. 



Figures 51-54: Mechanical rupturing of the cell; occurred 

 when smear was made or when it ivas blotted. 



51 Pulling apart of a cell that already had its nucleus at 

 one pole prior to pinching off an erythroplastid. 



52 Partial rupture of a cell in which nucleus lies near one 

 pole. 



53 Fracture of the cytosome. 



54 Partial squashing of the nucleus and fracture of the 

 cytosome. 



Figures 55-57: Extrusion of nuclei, found in a bird pre- 

 viously inoculated with lymphoid tumor cells. Manifes- 

 tation of the technic artifact probably enhanced by the 

 disease condition. 



55 Condensation of basichromatin. 



56 Shifting of the basichromatin outside the nucleus. 



57 Complete displacement of basichromatin outside the 

 nuclear membrane. 



Figures 58-60: Smudged cells. Fragile cells broken at the 

 time the smear loas made. 



58 Partially ruptured cell with squeezing out of liquid 

 basichromatin. Later stage shown in figure 2, 6. 



59 Partially ruptured cell with early chromophobic 

 reaction of the nucleus. 



60 Partially ruptured cell in which the nucleus was 

 almost completely chromophobic. 



Figures 61-63: Cells showing varying degrees of non- 

 refractile vacuolization of the cytosome. 



61 A few vacuoles of varying size lateral to the nucleus. 



62 Half the cytosome filled with large vacuoles. 



63 Small vacuoles filling the entire cell. 



Figures 64-69: Artifacts due to overheating the slide. 



64 — Heating has produced a few scattered refractile 

 vacuoles in the cytosome. 



65 Large and small refractile vacuoles in the cytosome. 



66 Coalescence of refractile vacuoles. 



67 A single large refractile vacuole at one pole of the 

 nucleus. 



68 Effect of excessive heat. Substance of refractile 

 vacuoles driven off leaving empty spaces. 



69 Staining of serum granules in an overheated slide. 



Figures 70, 71: Artifacts due to parts of smudged cells 

 falling on top of normal cells. 



70 Vacuoles and chain of three bodies beside the nucleus 

 due to overlying smudged nucleus. 



71 Two cells with a smudged nucleus overlying both of 

 them. 



72 Serum granules which have taken the stain. Compare 

 with figure 322. 



36 



