Chapter ^"g«" 



7 Technics for avian blood 222 



The microscope and light 222 



Procuring blood 225 



Preparation of cannulas for taking blood 



from early embryos 225 



Method for taking blood from the dorsal 



aorta of the 48-to-72-hour embryo. . . . 226 

 Method for taking blood from the heart of 



embryos of 96 hours and older 227 



The infrared lamp and its use in drying 



and warming slides 227 



Method for collecting and carrying blood 



samples 227 



Staining 228 



Selection, preparation, and use of Wright's 



stain 228 



Bulk staining with Wright's stain 229 



Mav-Griinwald Giemsa 230 



Chapter Page 



7 Technics for avian blood — Continued 



Staining — Continued 



Wright -Giemsa 230 



Petrunkevitch No. 2 and M. G. G 230 



Metln 1 alcohol and thionin 230 



Reticulocyte stain 230 



Ralph's modification of the benzidine tech- 



nic for hemoglobin in cells 231 



Miscellaneous technics 231 



Method for hemoglobin determination. . . 231 



Method for hematocrit determination. . . 232 



Method for making thrombocyte coimts. 232 



Method for white-cell coimts 232 



Literature cited 235 



Acknowledgments 243 



Index 245 



VI 



