H' 



^ 



-r^S^jt 



Hi 



Fig. 10. Mitochondrial membrane pellet. This pellet was obtained by centri- 

 fiiging a DOC-treated mitochondrial suspension as described by Watson and 

 Siekevitz (1956). There are no intact mitochondria, only single- and double- 

 membraned vesicles, some much smaller than and some nearly as large as 

 mitochondria. The mitochondrial matrix is gone, leaving optically-empty 



vesicular contents. 



Magnification = 20,000. (Watson and Siekevitz, 1956. Reproduced by 



permission of the Editors, J. biophys. biochem. Cytnl.) 



