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DISCUSSION 



Lipmann : I first used the term loose coupling in relation to thyroxine. 

 In our early experiments with Niemeyer and Kennedy, we found this 

 strange absence of the acceptor effect in mitochondria taken out from 

 thyroxinized animals. Then we stated that because in these preparations 

 we still could get P/O ratios which were normal, we like to consider this, 

 together with an absent acceptor effect, as a loose coupling (Hoch and 

 Lipmann, 1954, loc. cit.; Lipmann, F. (1956). In Enzymes: Units of 

 Biological Structure and Function, p. 444, Ed. Gaebler, O. H. New 

 York: Academic Press). I am very pleased that you have given it a 

 fitting explanation now. I called it loose coupling without really knowing 

 what I was talking about, but you have made some sense of it. 



Lehninger : Your experiments were a guide to us ; it was evident that 

 there had to be some kind of factor that caused this phenomenon. Very 

 few people have investigated this with uncoupling agents. It could be 

 concerned in the hyperthyroid condition. 



