218 E. Racker and R. Wu 



considerable importance for the physiology of the tumour 

 cell. Since this is not the only feature characteristic of tumour 

 cells, it becomes unimportant that some "normal" cells, such 

 as the leucocytes (which do not divide) also have a high 

 aerobic glycolysis. Whether or not one wishes to accept the 

 thesis that a damaged respiration is the primary lesion which 

 leads to increased glycolysis, one can only be impressed by 

 the universality of the high aerobic tumour glycolysis, which 

 must exert a decisive effect on all the other aspects of inter- 

 mediary metabolism. 



Summary 



A systematic investigation of the pacemaker of glycolysis 

 in ascites tumour cells revealed that the availability of 

 inorganic phosphate limits the rate of both lactic acid pro- 

 duction and glucose uptake. The controlling effect of inorganic 

 phosphate on glucose uptake is indirect due to limitation of 

 the glycolytic ATP regeneration, since ATP formed during 

 oxidative phosphorylation is not as efficiently utilized for 

 glucose phosphorylation. The Pasteur effect can be inter- 

 preted in terms of the decreased availability of inorganic 

 phosphate under aerobic conditions and the subsequent 

 diminution of glycolytic production of ATP. 



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