Role of TPN in Control of Glycolysis 



235 



Table I 



Phosphatase activities of liver mitochondria 



Each flask contained Tris-buffer pH 7 • 4 ; 3 [xm of substrate ; 3 jjiM-MgCla in a 

 total volume of 1 • ml. P = inorganic phosphate. 



ably unable to oxidize pyruvate or to bring about oxidative 

 phosphorylation (Siekevitz and Potter, 1955). Their G-6-Pase 

 contamination is weak or absent, but they have a considerable 



250 500 250 500 250 



AGED LIVER MITOCHONDRIA (mg.-equiv.) 



Fig. 2. Influence of variable amounts of aged mitochondria and 

 of TPN on glycolysis. Each flask contained in a 1 • 6-ml. volume, 

 0*5 ml. brain-Sg, variable amounts of mitochondria as indicated, 

 7-5 [jtM glucose, 1 -5 (XM ATP, 0-5 [xm DPN, 60 (xm nicotinamide, 

 6 txM-MgClg, 10 [JIM potassium phosphate pH 7-4, 1-5 \jm TPN 

 when indicated. 0-15 m-KCI to maintain isotonicity. 



ATPase activity (Table I). The addition of aged liver mito- 

 chondria modifies the rate of glycolysis by the brain super- 

 natant, as shown in Fig. 2, which also presents the effect of 



