114 GUIDE TO THE STUDY 



slightly alkaline by adding a few drops of 0.1 per cent sodium 

 bicarbonate. 



Grenacher's Borax-carmine. — Borax (4 per cent aqueous 

 solution), 100 cc; carmine, 3 grams. Boil until the carmine 

 dissolves, dilute with an equal volume of 70 per cent alcohol, 

 and filter after a day or so. Stain until penetration is complete, 

 often requiring days, and differentiate with acidulated alcohol. 

 For this 2 per cent of HC1 in 70 per cent alcohol is often used, but 

 better results are often obtained by using a weaker solution. 



Heidenhain's Iron Haematoxylin (see p. 99). — Be sure that 

 your mordant is made up from the clear violet crystals of the 

 ferric alum (iron-ammonium-persulphate). 



Wright's Stain. — This and the various other modifications of 

 Romanowsky's stain are difficult to prepare and are best pur- 

 chased from reliable dealers. Grtibler's powdered form of 

 Wright's stain has given us the best results. It should be 

 dissolved at the rate of 0.3 gram in 100 cc. of absolute methyl 

 alcohol (acetone free). For directions for use, see page 110. 



THE MORE IMPORTANT ENDOZOA OF LABORATORY ANIMALS 



The parasites discussed in the preceding outlines have been considered 

 in their systematic relationships without special regard to their source. As 

 an aid to the general examination of animals for endoparasites, the follow- 

 ing lists of the more important species infecting laboratory animals and of 

 the organs infected are given. 



The papers cited are in most cases comprehensive reviews or contain 

 important bibliographies. 



CAT 



PROTOZOA : 



Rhizopoda : 



Endamcrba histolytica, intestine. 

 Mastigophora : 

 Giardia felis (Giardia cati), intestine. 

 Trichomonas felis, intestine. 



felistomce, mouth. 

 Sporozoa : 



Eimeria felina, intestine. 

 Isospora bigemina, intestine. 

 felis, intestine. 

 rivolti, intestine. 

 Hepatozoon felis, leucocytes. 



