552 



TEMPERATURE INDUCED HETEROPLOIDY 



hauatlon of the reserve yolk at the third finger- bud stage), eind for the Anura some time 

 after the external gills have been resorbed. 



LATEBAL LINE 

 ORGANS 



PRIUOROIA OF 

 LATERAL LINE SYSTEM 



RED BLOOO 

 CELLS 



BLOOO ISLAND 



Dia;i,raramatlcal drawing, of a salaman- 

 der larva to show the origin of the 

 rudiments of the lateral line organs 

 and of the red Dlood cells which are 

 present in the cailtip. The ampu- 

 tated tailtip includes cells from 

 widely separated regions of the 

 embryos . 



From Fankhauser I958: 

 Proc. Am. Philosoph. Soc, 79:715 



Fix the tall tip In Bouln's fluid (it is not necessary to kill the larva) after clip- 

 ping It off about 1/5 the distance from the tip to the base of the body. After about h 

 hours, wash In the usual manner (decolorize in 0.51^ NHj^OH), wash in running tap water to 

 neutralize the tissue, and then stain for 7 to 10 minutes in about 531^ Harris' acid 

 haemalum (freshly acidified). Blue In tap water (alkaline), then dehydrate in the usual 

 manner, mount, and study. The percentage of cells showing chromosome figures will be 

 small, best in Triturus vlrldescens and poorest in the Axolotl and Eana. Drawings and 

 photographs of chromosome groups constitute the record, in addition to which drawings and 

 photographs of haplold, diploid, and trlplold larvae, their chromatophores and relative 

 cell sizes would be convincing (See figures on following page). 



PHOTOGRAPHS OR DRAWINGS OF TAIL TIP CHRCMOSOMES 



