316 



REGENERATION 



Anesthetize the larvae In l/ 10, 000 MS 222 and make the following cuts: 

 a. Cut off the right forelimh at ;he level of the wrist. 

 t. Cut off the right forelimb halfway between the wrist and the pectoral 

 gli^le, above the bend of the elbow. 



c. Repeat the above two cuts but make them at the greatest possible angles. 



d. Make the cuts described in "a" and "b" but extend the cut only half way 

 through the limb {or wrist), leaving all other parts attached. 



^^- 



Left forelimb of advanced Amblystoma, 

 indicating possible levels of amputa- 

 tion. 



(From Schotte' & Butler I9I+I: 

 Jour. Exp. Zool, 87:297) 



RECOED OF LIMB REGENERATIONS IN AMBLYSTOMA OB TBITUBU5 



