REGENERATION 315 



Quickly excise the anlage to be transplanted and insert it beneath this flap of 

 hlastema tissue, orienting it so that its ectodermal layer is outermost. Ee- 

 place the flap of blastema ectoderm and hold it down for 1 to 2 minutes with a 

 hall tip. The sticky blastema cells will become attached to the transplant and 

 will hold It in place. The transplant may be fixed any time from 18 hours to a 

 month after the transplantation, sectioned and studied to determine the degree 

 of differentiation and induction. 



SKETCHES OF OEGAN MLAGE TRANSPLANTED TO TAIL BLASTEMA 



LIMB REGENERATION 



The Urodelee show regenerative powers throughout life but the Anura exhibit them 

 until metamorphosis and then only to a limited degree thereafter. It is Instructive to 

 study the regeneration of limbs and digits of Urodele larvae. It must be remembered, how- 

 ever, that the post-operative larvae must be treated normally with respect to food, oxygen, 

 space, etc. If x-ray facilities are available, the effect of such irradiations upon re- 

 generating limbs can be studied (see Butler, 195lt and Butler & Puckett, 19^*0, and illus- 

 trations on the following peige). 



Procedure : 



1. Select Amblystoma or Triturus larvae beyond stage #38, when the limbs are 



developing or are already developed. It is essential that larvae of the same 

 age and stage be used in order to determine the effect of cuts at various levels 

 and angles. 



