EYE FIELD OPERATIONS 



295 



2. Prepare host larvae 'by excising the ectoderm, mesoderm and some of the under- 

 lying yolk on the lateral belly region of stage ^h or #25 ( see figures below) , 

 The prepared wound should "be slightly larger than the prospective graft. 



5. On the point of a needle transfer from the stained donor 



a. The right optic anlage 



b. The median transverse neural fold and medullary plate 



c. The entire (anterior) optic anlage (A-C-B in figxire above) 



on the prepared host sites. Hold in place with Briicke, or by hand, for a few 

 minutes until the graft becomes adherent. The vital dye will indicate the 

 limits of the graft. 



Scliema of a typical experiment, showing the re- 

 lations of the transplanted median and left 

 lateral strips of neural plate and their orien- 

 tation in the hosts. R, right; L, left. 



From Adelmann 1950: Jour. Exp, Zool. 57:225. 



By recording the exact shape of the graft at the time of excision, it will 

 be possible to record the axial relations of the graft in the host site. 



DRAWINGS OF ANLAGE TRANSPLANTATIONS 



