LIMB F lELD OPERATIONS 



285 



REGENERATION OF THE UROOELE LIMB 



Newly developing limbs of Urodele larvae have remarkatile powers of regeneration. 

 Amblystoma or Tri turns larvae with limbs (stage #38 or older) should be anesthetized in 

 1/5,000 MS 222 and the digits and limbs cut at various levels and angles, and be allowed 

 to regenerate. The following factors should be considered: 



a. Whether regenerative potencies can be eliminated by repeated extirpations. 



b. Whether the regenerated portion is structurally identical with that extirpated. 



c. Whether the level of the cut is the controlling factor in degree or perfection 

 of regeneration. 



d. Whether regeneration is achieved equally in limbs previously transplanted to 

 orthotopic or heterotopic positions. 



e. Whether limb regeneration is controlled by the associated girdle or nerve ele- 

 ments. 



In order to answer some of the above questions, it will be necessary to acquaint your- 

 self with the normal development and morphology of the limb and digits of the salamander. 



ng.2 



ANTERIOR LIMB AND GIRDLE OF AMBLYSTOMA PUNCTATUM 



Fig. 1. Mpdlal view of tlie skeleton of the left anterior 

 limb and girdle of Amblystoma punctatum, showing 

 the areas of attachment of the muscles (length 

 44 mm.) . 



Fig. 2. Lateral view of the skeleton of the left anterior 

 limb and girdle of A. punctatum, showing the areas 

 of attachment of the muscles (length 44 mm.). 



ABBREVIATIONS USED IN FIGURES 1 AND 2 



C, centrale 



Ce, carpale 



Co, coracoid 



H, humerus 



I, intermediujn 



Lat. Ep. , lateral epicondyle 



M, metacarpale 



Med. Ep., medial Epicondyle 



PCo, procoracold 



Ph, phalanges 



Proc. Lat., lateral process 



R, radius 



Re, radlale 



Sc, scapula 



SSc, suprascapula 



U, ulna 



Ue, ulnar e 



From I. W. H. Blount 1955: Jour. Exp. Zool. G^-.kO'J 



