INDUCED BREEDING 



105 



FLOOR OF CRANIUM DEFLECTED 

 FORWARD 



ENDOLYMPHATIC 

 TISSUE 



ANTERIOR PITUITARY GLAND OF FROG 



Diagram showing position of tlie pituitary 

 gland of the frog as it might be seen 

 through the parasphenoUal bone, lying 

 Just beneath the brain. 

 A-Anterior lobe of the pituitary gland. 



Note its more posterior position. 

 13-Pars intermedia and pars nervosa. 

 E-Eye ball as seen through oral skin. 

 Lr-Endolymphfitlc tissue adherent to the 



pituitary. 

 0-Exoccipital bone. 

 P-Parasphenoidal bone. 



R-Retractor bulbi muscle. The attachment of 

 this muscle to the parasphenoldal bone 

 must be partially removed. 

 S-Levator anguli scapuli muscle. 

 V-First vertebra. 



tissue. Occasionally the gland will remain adherent to the floor of the brain 

 case and will have heen deflected forward with the bone. Hemove the gland by 

 grasping the white endolymphatic tissue surrounding it, using sharp forceps. 

 This endolymphatic tissue has no known endocrine function. Place the gleini in 

 1 to 2 cc. of water in a small stender. In a similar way proceed with the re- 

 moval of as many glands as are necessary. Pituitary donors need not be freshly 

 caught animals but they must be sexually mature and in a pre-breeding condition. 

 In general, the female glands are about twice as potent as those from the male, 

 but there is no qualitative difference. The suggested doaes for Bana pipiens are 

 as follows : 



September to January 

 January to February 

 March 

 April 



Male Pituitaries 



10 

 8 

 5 

 1^ 



Female Pltultarlea 



5 

 h 



5 

 2 



In.lection of the hormone : For leclplents, carefully select large and obviously 

 healthy females which have been recently received from hibernation. Such females 

 may be kept In the refrigerator at k'-'C. in a small amount of water for a number 

 of weeks but should not be kept at laboratory temperatures for more than a week 

 if they are to be used for ovulation induction. Healthy females received from 

 hibernation in January may thus be kept until June (i.e., at 4°C. ) . At the labo- 

 ratory temperatures the ovarian eggs deteriorate rapidly. 



Before attaching the needle to the hypodermic syringe, draw up into its bar- 

 rel the requisite number of anterior pituitary glands. It will be best if the 



