EXPERIMENTAL EQUIPMENT AND PROCEDURES 13 



NUCLEAR STAINS 



A. Iron haematoxylln 



B. Feulgen, nucleal reaction 



C. Earris' acid haemalum 



12. Miacellaneoua reagents : 



A. Iodine - saturated iodine In 70^ (to follow Zenker's fixation). 



B. Ammoniated alcohol - % NBij.OH in IQ^ alcohol for removal of picric. 



C. Glacial acetic acid - and Normal acetic (60 cc. Glacial/liter). 



D. I^ydrochlorlc acid - concentrated, 1^ and Normal. 



E. Sodium hydroxide - concentrated, 1^, Normal and O.OOJN. 



F. Ammonium hydroxide - concentrated. 



G. Potassium hydroxide - 1^. 



H. Bydrogen peroxide - concentrated (can be used as 'bleaching agent). 



I. Acetone 



J. Glycerine 



K. Cleaning fluid (K. Bichromate and sulphuric acid). 



L. Clearing agents : 1. Xylol (toluene, toluol, benzene (benzol) 



2. Cedar oil 



3. Aniline oil 



k. Oil of wlntergreen (methyl salicylate) 



5. Oil of cloves 



6. Dioxan (volatile and presumably toxic) 



7. Chloroform 



M. Mounting media : 



N. Embedding media : 



1. Canada balsam - dissolved in xylol 



2. Gum damar 

 5. Clarlte 

 k. Egg albumen - best as albumen water (3^) 



1. Paraffin : M.P. range from U5OC. to 58°C. 



2. Beeswax: pure white 



' 5. Bayberry wax (Candle Factory, Falmouth, Mass.) 



k. Rubber - white rubber to be added to paraffin 



0. Vaseline 



P. pH indicators (LaMotte sets for entire range) 



MISCELLANEOUS EQUIPMENT 



A. Operating base 



1. Permoplast - American Art Clay Company, Indianapolis, Indiana. 



2. Rainbow wax - 



3. Beeswax with lampblack to give proper background shade. 

 h. Board with cut-out to fit over binocular base. 



B. Lampblack 



C. Agar 



D. Cellophane, pliofilm or pyralin . 



E. Lucite - used to conduct light for considerable distances, without heat 



transmission. Can be used to curve light and point it Into operating dish. 



F. DeKhotinsky's cement - for mounting razor blade fragments onto glass handles. 



G. Paper 



1. Toweling 



2. Lens 

 5. Filter 



H. Marking devices 



1. China marking pencils: red, blue or black 



2. Diamond point 



5 . Carborundum mounted on glass rod 



1. Cloth 



1. Cheese 



2. Black velvet (to reduce movement of ciliated larvae) 



