12 EXPERIMENTAL EQUIPMENT AND PROCEDURES 



Killing and fixing aolutlona (Continued) 



G. Gllaon's fixative - Camoy & Lebrun, l897: La Cellule 12: 



Nitric acid - 80^. 15 cc. 



Glacial acetic acid_ k cc. 



Corrosive sublimate 20 grams 



Alcohol 80^ 100 cc. 



Water - distilled 880 cc. 



H. Gatenby's fluid : 



K. Bichromate - 2% aqueous 100 cc. 



Chromic acid 1^ 100 cc. 



Nitric acid 6 cc. 



I. Stockard's solution - for fish embryos: 



Formalin 5 pta. 



Glacial acetic k pta. 



Glycerine 6 pta . 



Water 85 pts . 



J. Acetic alcohol: 



Absolute alcohol 90 cc. 



Glacial acetic 10 cc. 



K. CoiTosive acetic : 



HgClg 5 gr . 



Glacial acetic 10 cc. 



Water 90 cc. 



L. Formalin - two concentrations should be available, 10^ and h'^. 



11. Bleaching Agents : 



A. Javelle water - potassium hypochlorite. 



B. Mayer's chlorine - to be made up Just before use 



1. Place few crystals potassium chlorate in vial. 



2. Add 2-5 drops HCl. 



5. When green chlorine fumes evolve, add 2-10 cc. of 70^ alcohol. 

 h. Transfer specimens from pure 70^ alcohol to this mixture until 

 bleached. 



C. Amitinnlated alcohol - 2^ NHj^OH in 70^ alcohol to decolorize picric acid stain. 



CYTOPLASMIC STAINS 



A. Eoaln - O.J^ in 95^ alcohol. 



B. Light green (Griibler'a) - 0.5^ in 95^ alcohol. 



C. Fast green (Nat. Aniline Co., N.G.f.5) 0.5^ in 95^ alcohol. 



D. Safranln (Nat. Aniline Co., N.S.-IO) 1^ in aniline water. 



E. Orange G - sat. in clove oil. 



F. Maason stains (A,B,C.) Excellent for cell types (pituitary). 



G. Alizarine S - (used in Spaltoholz technique). 



VITAL DYES 



A. Nile blue sulphate - 1/200,000 



B. Methylene blue - 0.5^ 



C. Neutral red - 1^ 



D. Bismarck brown - 1^ 



E. Janua green - 1^ (aee section on "Vital Staining" etc.) 



