10 EXPERIMENTAL EQUIPMENT AND PROCEDURES 



GLASSWARE 



1. Battery .lara or aquaria for individual use of students; each with weighted wire 



screen cover. 



2. Crystallizing;^ dishes or 10 inch finger bowls. 



5. Finger bowls - regulation size, made to fit Into each other. 



k. Petri dlahea (10 cm.) with covers. 



5. Stender dishes with covers, both #1 and #2. 



6. Syracuse dishes - regulation size. 



7- Coplin staining .lars and Homeopathic and Shell Vials . 



8. Salt cellars for embedding oven. 



9- Erlenmeyer flasks - 500 cc. capacity for storing sterile media. 



10. Lantern slide covers - used as glass base for operating and for protecting binocu- 



lar and compound microscope stages when using wet mounts. 



11. Graduates - 10 cc. and 100 cc. 



12. Beakers - 100 cc. and 600 cc. 



15. Slides : Begulation microscope slides. 

 Depression slides, cell type. 

 Ik. Cover slips - glass. 'Best size, 7/8 Inch square and #1. 



15. Qlaaa rods - Diameters from 1+ to 7 mm- Soft glass. 



16. Glass tubing - Diameters from 4 to 7 mm. Soft glass. 



SOLUTIONS AND REAGENTS 



1. Distilled water - glass distilled preferred. Supply in large carboys with siphon 

 and pinch clamp. 



2. Spring water - if possible supply in carboys with siphon and pinch clamp. Great 

 Bear Spring Water (N. Y. City) is excellent. 



5- Conditioned tap water - to be stored in carboys after conditioning. This will be 

 necessary where the City water supply la treated ao that embryoa cannot survive 

 in it. This may be tested with sperm or early embryos. Conditioning is achieved 

 by running tap water into large tank in which there will be maximum of surface 

 exposed and in which plant material la abundant. Artificial aeration will help to 

 eliminate chlorine. Paaslng tap water through fine gravel and charcoal la rarely 

 necessary but would aid in conditioning highly toxic water. Three or four days 

 of such conditioning should be sufficient. 



h. Standard (Holtf reter' a) Solution - this should be available to the students in 

 several concentrations and large volumes. For the convenience of the instructor, 

 the dry salts may be made up in appropriate concentrations and stored in vlala to 

 be added to carboys of glaaa diatilled water when needed. The concentrations 

 needed are 200^, 100^ and 205^. The formula for Holtfreter's solution (J. Holt- 

 f reter, I93I - Arch. f. Ent. mech. 12l*^:Uol*) is: 



NaCl 0.55 gr. 



KCl 0,005 gr. 



CaClp 0.01 gr. 



NaHCbj 0.02 gr. 



Water 100.00 cc. (glass distilled preferred) 



5. Amphibian Binder's - should be available In concentrated form: 



NaCl 0.66 gr. 



KCl 0.015 gr. 



CaCl^ 0.015 gr. 



NaHCOj to pH 7-8 



Water 100.0 cc. 



6. Urodele stock solution: 



Great Bear Spring Water 10 liters 



NaCl 70 grams 



KCl „ 1 gram 



CaClp 2 grama 



