EXPERIMENTAL EQUIPMENT AND PROCEDURES 



5. Glaaa 'ball tip : This Instrument la also used for moving embryos about without in- 

 Jury to them. Using 6-7 mm. soft rod of about 18 cm. lengths, draw out the center 

 (flame tip) and break off the excess thread down to within 2-3 cm. of the widening 

 part of the rod. Hold the pointed end of the rod in a flame and a glass ball will 

 form on the end. It can be kept symmetrical by constant spinning of the rod while 

 heating. If it is planned to put a gentle curve in such a rod, this should be 

 done when the rod is drawn out rather than later. Such ball-tips are used also 

 for making depressions in Permoplast, suitable for embryos. It is well, there- 

 fore, to have a small assortment of such ball-tips. 



6. Glass pipettes : Three types of pipettes will be used: wide-mouthed, micro- 

 pipette, and micro-pipette with lateral control (see diagrams). 



Y 



V 



\i 



GLASS TUBING 



MICRO - BURNER 



GLASS BALL TIPS // 



MICRO - NEEDLES 



\J u 



HAIR LOOPS 



@nl 



FINGER COrfTfiOL 

 OF TERMINAL SUCTION 



r® 



•- SAFETY PIN 



HOLE IN SIDE OF PIP£TTE 



TXIN RueSER GUARD 



TRANSFER PIPETTE 



2 MM OPENING 



Ij V PTRALIN • TRANSPARENT SRUCKE 



BRUCKE TO HOLD TRANSPLANT IN PLACE 

 ( STULTZ '38) 



The wide-mouthed pipette is used for the transferring of embryos. It Is 

 aimply a wide bore pipette (6-7 mm. diameter) with a curved and amooth-edged tip. 

 Thia should be available at all times. 



The micro-pipette is made of soft glass with 7-8 mm. outer diameter, pulled 

 out to microscopic dimensions in a manner similar to the making of micro-needles 

 (above). The tip end of such pipettes are often closed but may be trimmed off. 

 These pipettea may be uaed with ordinary rubber nipple or may be uaed with rubber 

 tubing and mouth suction. 



The micro-pipette with lateral control has special use in the transfer of 

 isolated pieces of embryoa to regions where transplants are desired. It is made 

 in the following manner: Pull out some 7-8 mm. soft glass tubing so that the 

 handle portion has a length of about 10 cm. It is better if the capillary end has 

 a alight curve and tapers rapidly. Close the capillary end by melting it in the 

 Bunaen flame and attach a piece of rubber tubing to the larger open end of the 

 pipette. Bring the aide of the pipette, at about ^-h cm. above the capillary end, 



RUBBER TUBING OVER 

 SIDE HOLE 



Spemann Mioropipette for Transplantations 



