BREEDING HABITS OF AMPHIBIA 



111 





Typical egg clusters of 

 spotted salamander as they 

 appeared on April 9. A 

 spermatopliore can be seen 

 on leaf below large clus- 

 ter. (After Bishop) 



Spermatophores of spotted salamander in situ 

 on April 14. Inset: A single one, greatly 

 enlarged. (After Bishop) 



(Courtesy C. H. Pope I9UI4.: 

 Chicago Mua. Nat. Hist.) 



These eggs will tolerate refrigerator temperatures as low as i+°C. and if kept at such 

 low temperatures the time to gaatrulatlon may he extended to ahout 10 days. The optimum 

 temperature for development is between, 12°C. and 15°C., hut the embryos will tolerate 

 20°C. at the upper limit. At 10°C. the embiyos pass from stage #7 to stage #25 in about 

 500 hours (13 days). These are excellent eggs and embryos for operative procedures. 



AMBLYSTOMA TIGBINUM , the tiger salamander. This salamander is found throughout the United 

 States and In Canada and Mexico. There are numerous yellow spots which cover the body but 

 are largely concentrated along the sides of the belly, In contrast with A. punctatum. The 

 background color la deep brown to black and this is the largest of the salamanders except 

 the mudpuppy and siren. It measures about 10 inches in length. The males are the larger 

 sex and their vents protrude. 



Mature larva of tiger salamander. 



(Courtesy C. H. Pope I9UU: 

 Chicago Mus. Nat. Hist.) 



Amblystonia tigrlnum, 

 the tiger salamander. 



Amblystoraa punctatum, 

 the spotted salaman- 

 der. 



