TABLE OF CONTENTS 



Pa^e 



Frontispiece 



Introduction - ..Hi 



Eeference Books for Experimental Embryology — - vi 



TECHUIQUES IN EXPERIMENTAL MBRYOLOCT 



Equipment and Procedures in Experimental Embryology 1 



Technique for Staining Chromosomes in Tail-fin 26 



THE NORMAL DEVELOPMENT OF AMPHIBIA 



Notes on the Breeding Hahits of Some Common Amphlhla _ 30 



The Culture of Amphibian Embryos and Larvae to Metamorphosis - '•8 



The Developmental Stages of Amphibian Embryos .._ — _ 56 



Eana pi pi ens - 57 



Xenopus laevis - — - 90 



Hyla regllla _ 92 



Ambly stoma punctatum _. 9'*' 



Trlturus pyrrhogaster — 100 



Trlturus torosue ~ 101 



Induced Breeding 102 



Ovulation and Egg Transport 11** 



Early Behavior Patterns In the Amphibia 121 



EXPERIMENTS ON EARLY DF-^LOPMENT - NON- OPERATIVE 



Fertilization of the Frog's Egg 126 



The Effect of Age of the Egg on Embryonic Development 150 



Osmo- regulatl on ' 153 



Temperature and Embryonic Development ^ — 137 



The Space Factor and the Rate of Growth and Differentiation l't-6 



Nutrition and Growth of Amphibian Larvae 152 



Mechanical Separation of Growth and Differentiation 157 



Chemical Alteration of Growth and Differentiation l62 



The Embryo and Narcosis, or The Separation of Form and Function - 166 



EXPERIMENTS ON EARLY DEVELOPMENT - OPERATIVE 



Experiments with the Amphibian Germinal Vesicle - 171 



Androgenesi s 179 



Artificial Parthenogenesis 1"5 



Pressure Effects on Cleavage - 190 



The Effect of Centrlfugatlon on Development - 193 



The Production of Double Embryos - - - — 198 



EXPERIMENTS ON THE EARLY EMBRYO 



The Behavior of Isolated Embryonic Cells 203 



The Organizer and Early Amphibian Development — ~ 210 



Morphogenetlc Movements as Determined by Vital Staining 218 



The Culture of Isolated Amphibian Anlagen 228 



Wound Healing in Embryos - 255 



. 2M-0 



Parabiosis and Teloblosls 



Extirpation Experiments on Organ Anlagen •— 214-5 



Transplantations — — - - 255 



The Origin of Amphibian Pigment ~ — . — ~ 263 



