EXPERIMENTAL EQUIPMENT AND PROCEDURES 



EQUIPMENT AND INSTRUMENTS NEEDED 

 BY EACH STUDENT IN EXPERIMENTAL EMBRYOLOGY 



OPTICAL EQUIPMENT 



Blaocular microscope and llshtin^ equipment 



Objectives 1.7 x 3'5 x most useful, with lOx oculars. 

 The U-shaped base and mirrors should be removable. 

 Microscope lamp: Spencer diaphragm type most suitable. 



Heat absorbing flask: 250 cc. round bottom Pyrex flask filled with distilled 

 water and supported by screw clamp to vertical stand. 



GLASSWARE 4 CULTURE DISHES 



2 rectangular enamel pans Js x 12 x 2k Inches (for tadpoles) 



2 battery Jars with weighted wire covers 



2 crystallizing dishes with covers, 12 Inch size 

 12 Syracuse dishes 

 12 #1 Stenders with covers 

 12 #2 Stenders with covers 

 12 regulation finger bowla 



6 pairs Petri dishes 



6 shell depression slides 



i gross microscope slides 



J ounce #1 cover slips, square or round 



2 ft. glass tubing, 5 nmi. diameter (soft glass) 



2 ft. glass rod, k mm. diameter (soft glass) 



h ft. glass rod, 2 mm. diameter (soft glass) 



1 hypodermic syringe (2 cc. capacity) with #l8 needles 



METAL INSTRUMENTS AND EQUIPMENT 



2 watchmaker ' s forceps #5 



2 adjustable needle holders 



1 fine-pointed, sharp scissors (iridectomy scissors are expensive but useful) 



1 heavy-duty scissor 



2 fine, sharp scalpels, one a lancet type 

 1 rigid section lifter 



1 pair regulation forceps 

 1 triangle file 



1 Bunsen burner with wire gauze. 



MISCELLANEOUS 



i lb. Permoplaat, cream colored 



r- lb. beeswax, white, clear 



^ lb. soft melting point paraffin 



2 China marking pencils, red and blue 

 1 diamond or carborundum point pencil 



1 wooden needle holder, to hold needles of various sizes 



1 celluloid cover for needles and holder 



2 ft. rubber tubing 11 or 12 mm. outer diameter 

 2 ft. rubber tubing 8 or 9 n™- outer diameter 



THE PREPARATION OF INSTRUMENTS 



1. The micro -burner : This will be set-up for class use but it is well that a de- 

 scription be given here for its preparation. The function of a micro-burner is 

 to give a small but intense flame. 



