VITAL STAININ G AND MORPHOGENET I C MOVEMENTS 22? 



It is possible to preserve the Nile "blue sulphate for histological examination by us- 

 ing the following procedure: (Stone, 1952) 



a. Fix in Zenkera-acetic for 2 hours. 



b. Wash in tap water. 



c. Place in I'jt Phosphomolybdic acid for 2 hours. 



d. Transfer to dioxan to which 0.1'?& Phosphomolybdic acid has been added - for 

 2 two-hour changes. 



e. Transfer to cedar oil plus 0.1'^ Phosphomolybdic acid until clear. 



f . Embed in paraffin, 5 baths of about 15 minutes each. 



g. Section and mount in xylol-clarite. 



(It is the Phosphomolybdic acid which keeps the Nile blue sulphate in position.) 



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Adams, A. E. , I928 - "Paraffin sections of tissue supravitally stained." Science, 68:305. 

 Bankl, 0., 192? - "Die Lagebeziehungen due Spermium-Eintrittsstelle zur Medianebene und 



zur ersten Furche, nach Versuchen mit orlichen Vitalfarbungen Axolotei." Anat. Anz. 



65:196. 

 Carpenter, E,, I957 - "The head pattern in Amblystoma studied by vital staining and trans- 

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 Conn, H. J. & B. S. Cunningham, 1952 - "The use of dyes as vital stains." Stain Technology. 



7:81. 

 Cunningham, E. 8. Sc H. J. Conn, 1932 - "Methods for the preservation of supravitally 



stained material." Stain Technology. 7:15. 

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Proc. Soc. Exp. Biol, & Med. 37:380 (See Detwiler, I917, Anat. Bee. 13:'+95). 

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cens." Jour. Exp. Zool. 85:l6l. 

 Goerttler, K., I925 - "Die Formbildung der Medullaranlage bei Urodelen. Im Bahman der 



Verscheibungsvorgange von Keimbezirken wahrend der G"8trulation und ala entwicklungs- 



physiologisches Problem." Arch. f. Ent. mech. 106:505. 

 Harvey, E. B., I9I+I - "Vital staining of the centrlfuged Arbacia punctulata eggs." Biol. 



Bull. 80:lll+. 

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young Amphibian gaatrula (Amblystoma and Disfcoglaaaus) . " Jour. Exp. Zool. 289:255. 

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