CONTENTS 



Mechanism of Cell Division 

 Polar-body Emission 

 Cleavage of the Fertilized Egg 

 Fragmentation of Eggs 



72 

 73 

 78 

 84 



Membranes and Investments 



Vitelline Membrane 



Zona Pellucida .... 



Cumulus Oophorus 



Mucin Coat of the Rabbit Egg . 



Outer Coats of Marsupial and Monotreme Eggs 



86 



89 



96 



100 



102 



MANIPULATION OF EGGS 



Microscopy . . . . . .103 



Transfer ....... 109 



Studies on Eggs Maintained in vitro : Metabolism — ■ In- 

 fluence on spermatozoa — Resistance to low tempera- 

 tures — - Development in culture — Fertilization /// vitro 1 1 1 



