80 G. W. SALISBURY 



Release of pC0 2 Inhibition by Sulfite and Its Control by SH 



In Table VII is shown the first of these conditions. Sulfite in 

 amounts comparable to the levels found in semen releases a consider- 

 able part of the inhibition of anaerobic glycolysis clue to 100% pC0 2 

 (Salisbury, 1959). This release by sulfite is in turn inhibited by an 

 equivalent sulfur level in the form of sulfhydryl radical of cysteine. 

 Other data on this problem are shown in Table VIII (Lodge and 



Table VII. Inhibition of anaerobic glycolysis by pC0 2 , its partial release 

 by sulfite, and control of the release by sulfhydryl radical, in Na citrate- 

 bicarbonate diluent during incubation of 4 hours at 37°C 0> b 



Gas 



100% N 2 25% C0 2 100% C0 2 



( M l CO2 evolved by 10 s cells in 4 hr) 



Control, I VT N ./ 213.5 81.8 2.8 



IVT Na + sulfite (30 t S/ml) 209.0 114.8 79.5 



I VT Na + sulfite 4- cysteine (30 1 98 . 5 79.5 4.8 

 7 S/ml for both) 



" n, number of semen samples, equals 4. 



h Lodge and Salisbury (1960). 



c IVT is the Illini variable-temperature diluent. 



Table VIII. Inhibition of anaerobic glycolysis by /;C0 2 , its partial release 

 by sulfite, and effect of free and bound sulfhydryl on the release in K cit- 

 rate-bicarbonate diluent during incubation of 4 hours at 37°C a 



a Lodge and Salisbury (1960). 

 6 All additions at 30 7 of S/ml. 



