IONIC AND OSMOTIC CONDITIONS 



77 



2 3 4 



TIME - HOURS 



Fig. 8. Anaerobic glycolysis, as measured by C0 2 evolution from cit- 

 rate-bicarbonate buffer, by spermatozoa in semen under N 2 when the pri- 

 mary cation is sodium or potassium. Number of semen samples, //, equals 

 3; IVT is Illini variable-temperature diluent (Lodge and Salisbury, 1960). 



tions, proving that the K+ is inhibitory under both aerobic and 

 anaerobic conditions, and possibly by its effect on ATPase as shown 

 in perch sperm flagella by Tibbs (1959). 



Similarly, Fig. 9 shows the results from use of the same diluents, 

 IVT K and IVT Na , on the anaerobic glycolysis of washed spermatozoa, 



ZoO^O 



0.5 ix) 



INCUBATION TIME 



Fig. 9. Effect of washing of spermatozoa and equimolar concentra- 

 tions of sodium and potassium in citrate-bicarbonate media on anaerobic 

 glycolysis under 5% C0 2 , 95% N 2 at 37 °C. Number of sperm samples, n, 

 equals 2; IVT is Illini variable-temperature diluent (Salisbury et al., 1960). 



