22 PIERRE H. GONSE 



— | 1 | I I I ll| 1 1 I I I I ll| — 1 1 I I I I l' | 1 I 1 ' I Ml 



200 



§150 



50 



Full mof. max. inh. — M no mof 



I I I III 



0.01 0.1 n - ™ mM azide 



Fig. 13. Effect of azide on respiration in states 1 and 3 shown for Spisula 

 spermatozoa. Cells in SW-GG, pH 7.3. Results from different preparations. 

 #, azide on endogenous respiration (Z . 2 of about 1); O, azide in the pres- 

 ence of DNP, 0.5 X m~ A M (Z 02 = 5 to 8). Within the frame, progressive 

 inhibition of motility. 



phenomenon is very different from that which occurs with other re- 

 spiratory inhibitors. Also different is the fact that addition of glu- 

 cose, absent from the seminal fluid of dogs, does not reverse the in- 

 hibition of motility. An action of azide on glycolysis therefore seems 



