124 



PIERRE H. GONSE 



100 



50 



DOG: Full moK 



max. mh. 



"ii| 



pari. mol\ 



0.1 



10 mM 

 amyfal 



Fig. 15. Effect of Amytal on exogenous respiration. # dog sperma- 

 tozoa, seminal fluid with GG, pH 7.3; Q, bull spermatozoa, washed and 

 suspended in RP, pH 7.2, lactate 20 mM. Within the frame, progressive 

 inhibition of motility. 



be responsible for a large part of the respiration of Spisula sperma- 

 tozoa. 



During inhibition of motility by Amytal, all cells are affected at 

 the same time and to the same extent. With increasing Amytal con- 

 centrations, one finds a progressive decrease in frequency of tail beat, 

 the wave traveling more and more slowly along the flagellum, while 

 amplitude remains unmodified. Addition of glucose completely re- 

 verses Amytal inhibition of motility in dog spermatozoa. Another 

 observation recorded in Fig. 16 is the greater sensitivity to Amytal of 

 DNP-activated respiration in Spisula sperm. This indicates a possible 

 change in the nature of substrates utilized as induced by DNP. 



With antimycin A, inhibition of motility of dog spermatozoa does 

 not parallel that of respiration (Fig. 17). Motility is affected at very 

 low concentration of the inhibitor, 2 X 10 _8 M. As seen previously, 



