RESPIRATION AND OXIDATIVE PHOSPHORYLATION 



127 



o 

 ± 6 



TTTTT 1 r 



-\ 1 l I I I 



Full moh 



max- ink 



no mof. SPISULA 



DOG : full moN 

 ■ ■ ■■■■ii i_j 



c, inh.^»[^^N^7 



parh mot. 



0.001 0.01 0.1 1. mM DNP 



Fig. 18. Effect of dinitrophenol on respiration of different sperma- 

 tozoa. ▼ , dog spermatozoa, seminal fluid with GG, pH 7.3; V, bull sper- 

 matozoa, washed and suspended in RP, pH 7.2, lactate 20 mM; % . clam 

 spermatozoa (Venus sp.), in SW-GG, pH 7.3; Q, Spisula spermatozoa, in 

 SW-GG, pH 7.3. Within the frame, progressive inhibition of motility, 

 for Spisula (top), for dog (bottom). 



tion of motility in dog spermatozoa, where "glycolytic motility" is 

 largely insensitive to oxidative metabolism and oxygen tension (Fig. 

 19). There seems to be no Pasteur effect on motility, a confirmation 

 of the data of Lardy et al. (1949) on lactic acid production by ejacu- 

 lated bull spermatozoa in aerobic or anaerobic conditions. However, 

 the reverse phenomenon, a control of oxidative motility by glycoly- 

 sis, is not unlikely in view of the fact that the presence of glucose 

 prevents the high response of motility to oxygen. This transient burst 



