174 



LEONARD NELSON 



SPECIFIC ACTIVITIES OF FLAGELLAR ENZYMES 



W - Total Suspension 



T - Tail 



M - Midpiece » 



H - Head 



ATP-ase 



Succinic 

 Dehydrogenase 



Cytochrome 

 Oxidase 



Fig. 1. Specific activities of bull sperm enzymes. ATPase, micrograms 

 phosphorus liberated from ATP per milligram of nitrogen (of suspension) 

 per minute. Succinic dehydrogenase, A log [Ferricytochrome c] per milli- 

 gram nitrogen per minute. Cytochrome oxidase, A log [Ferrocytochrome 

 c] per milligram nitrogen per minute. Enzyme activity is concentrated in 

 tail and midpiece fraction, negligible activity in head fraction shown for 

 comparison. Both reduction and oxidation of cytochrome c are relatively 

 uniform throughout flagellum. 



