CYTOCHEMICAL ASPECTS 183 



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Fig. 9. Myosin antibody reactivity of frozen-dried rat sperm, (a) In- 

 cubated in myosin antibody preparation 30 min; (b) control in normal 

 rabbit serum. (Not illustrated are specimens incubated in antibodies and 

 normal serum preparation after prolonged extraction with 0.6M KI and 

 other actomyosin and myosin extractants; after such treatment, there is 

 no longer any localized increase in electron opacity due to antibody ad- 

 sorption.) (x 15,000.) 



ishes the reactivity (Nelson, 1961) (Fig. 9). However, if similarly 

 extracted sperm are stained with PtBr 4 , the peripheral fibers may 

 still be seen, confirming the complex nature of the fibers suggested 

 by the previously mentioned investigations in sperm cytochemistry. 

 Burnasheva's report (1958) that extracted spermosin complexes with 

 muscle actin implies that spermosin more nearly resembles myosin 

 than actomyosin and that therefore sperm actually lack actin. While 



